OUR BRANDS ENSEMBLE, AIDONS LA SCIENCE À PROGRESSER TOGETHER, LET'S HELP SCIENCE TO PROGRESS
The Dutscher Group brands Dear customers, We are delighted to introduce you to the brands of the Dominique Dutscher Group, all gathered in this brochure. The Dominique Dutscher Group's brands cover a range of laboratory consumables (tips, filter tips, cryotubes, microtubes, etc.) and benchtop equipment with common benefits: • premium quality to the highest standards; • Dominique Dutscher's control of the manufacturing process and strict compliance with the specifications; • technical advantages: all ClearLine® products feature unique characteristics that provide additional safety and ease of use; • exceptional value for money. Please do not hesitate to contact us. For guidance and ordering Contact AH diagnostics for product support and ordering. 87 45 90 10 (0)8 680 0845 2323 3260 (0)10 325 3000 ahdiagnostics.dk ahdiagnostics.se ahdiagnostics.no ahdiagnostics.fi order@ahdiagnostics.dk order@ahdiagnostics.se order@ahdiagnostics.no order@ahdiagnostics.fi
Contents 1. CELL CULTURE P. 4-9 2. MOLECULAR BIOLOGY P. 10-29 3. SEROLOGY - IMMUNOLOGY P. 30 4. MICROBIOLOGY - ANALYSIS P. 31-35 5. STORAGE SCREENING P. 37 6. PIPETTING & DISPENSING P. 38 7. TIPS & MICROPIPETTES P. 39-42 8. MEDIA & CHEMICAL PRODUCTS P. 43-49 9. TUBES & RACKS P. 50-58 10. CRYOGENICS P. 59-61 11. PLASTICWARE P. 62- 63 12. FILTRATION P. 64-69 13. GLASSWARE P. 70 14. HEALTH & SAFETY P. 71-73 15. EQUIPEMENT P. 74-77
4 CELL CULTURE CELL CULTURE ClearLine® standard cell culture flasks z Polystyrene crystal bottles z Easy stacking z Packaged in peelable bags z Certificates available on request z Printing of batch number and expiry date on each bottle z Model with filter cap: with hydrophobic membrane, prevents the passage of bacteria, spores and particles larger than 0.2 µm Part no. Type of collar Type of cap Treatment Units/ bag Units/box €/ box 25 cm² surface area 131000C Inclined Standard TC 10 200 NC - 131006C Inclined Filtering TC 10 200 NC - 75 cm² surface area 131002C Inclined Standard TC 5 100 NC - 131008C Inclined Filtering TC 5 100 NC - 175 cm² surface area 131004C Inclined Standard TC 5 40 NC - 131010C Inclined Filtering TC 5 40 NC - Endo- toxins z In peelable bags z Sterilised by gamma rays z Certified endotoxin levels below 0.5 EU/ml z Certificates available on request ClearLine® Petri culture dish Part no. ext Ø x ext. h (mm) Treatment Units/ bag Units/ box €/ box Ø 35 mm 131046C 37.8 x 12.5 TC 10 960 NC - Ø 60 mm 131048C 58.8 x 17.8 TC 10 600 NC - Ø 100 mm 131050C 95.6 x 22 TC 10 300 NC - Ø 150 mm 131052C 143 x 21.9 TC 10 120 NC - Endo- toxins ClearLine® transparent multi-well plates and microplates z Polystyrene z Sterilised by gamma rays z Alphanumeric marking z Notched corners z Certified endotoxin level ‹ 0,5 EU/ml z With lid Part no. Number of wells Bottom type Treatment Units/ bag Units/ box €/ box 131028C 6 Flat TC 1 100 NC - 131024C 12 Flat TC 1 100 NC - 131020C 24 Flat TC 1 100 NC - 131019C 48 Flat TC 1 100 NC - 131012C 96 Flat TC 1 100 NC - Endo- toxins ClearLine® cell scrapers z Individual sterile packaging z ABS handle and TPE blade Part no. Handle length (cm) Blade length (mm) Packaging unit Units/ box €/ box 131054C 25 20 1 100 NC - 131056C 39 30 1 100 NC - Endo- toxins
5 Prices are indicative and excluding tax. Visit Dutscher.com CELL CULTURE CELL CULTURE z Sterile Part no. Pore size (µm) Colour code For tubes Pack. unit Units/ box €/ box 141378C 40 Blue 50 ml 1 50 NC - 141379C 70 White 50 ml 1 50 NC - 141380C 100 Yellow 50 ml 1 50 NC - ClearLine®Cell Strainers Endo- toxins Part no. Origin Vol. (ml) € S1810-100 South America 100 NC - S1810-500 South America 500 NC - S1650-100 Mexico, USDA approved 100 NC - S1650-500 Mexico, USDA approved 500 NC - S1520-100 USA 100 NC - S1520-500 USA 500 NC - S1710-500 New Zealand 500 NC - S1400-100 Ireland 100 NC - S1400-500 Ireland 500 NC - Fetal calf serum SVF Ultra low endotoxins SVF Premium Part no. Treatment Vol. (ml) € S181H-100 Heat inactivated 100 NC - S181H-500 Heat inactivated 500 NC - S181F-100 Carbon adsorption 100 NC - S181F-500 Carbon adsorption 500 NC - S181D-100 Dialysed 100 NC - S181D-500 Dialysed 500 NC - S181I-500 Depleted IgG 500 NC - S181M-500 Exosome Depleted 500 NC - Part no. Treatment Vol. (ml) € S181L-100 Lipid Depleted 100 NC - S181L-500 Lipid Depleted 500 NC - S181T-100 Without tetracycline 100 NC - S181T-500 Without tetracycline 500 NC - S181R-100 Iron supplemented 100 NC - S181R-500 Iron supplemented 500 NC - S181R-500 ES Cells 100 NC - S181R-500 ES Cells 500 NC - Fetal calf serum with treatment: South American origin Part no. Origin Vol. (ml) € S0250-500 Bovine (French origin) 500 NC - S0400-500 Calf 500 NC - S0400-1000 Calf 1000 NC - S0750-100 Newborn calf 100 NC - S0750-500 Newborn calf 500 NC - S0900-100 Donor horse 100 NC - S0900-500 Donor horse 500 NC - S0910-100 Horse 100 NC - S0910-500 Horse 500 NC - S0800-100 Donor Foal 100 NC - S2170-100 Donkey 100 NC - S2170-500 Donkey 500 NC - S2000-100 Goat 100 NC - S2000-500 Goat 500 NC - S2350-100 Sheep 100 NC - S2350-500 Sheep 500 NC - S2400-100 Pig 100 NC - S2400-500 Pig 500 NC - S0500-100 Hen 100 NC - S0500-500 Hen 500 NC - S2500-100 Rabbit (French origin) 100 NC - S2500-500 Rabbit (French origin) 500 NC - S2150-010 Rat 10 NC - S2150-100 Rat 100 NC - S2160-100 mouse 100 NC - Animal serum z Reproducible from batch to batch z Growth > 80% z Saves time, without testing z Non-samplable z Endotoxin levels< 5 EU/ml z Hemoglobin levels < 25 mg /100 ml z South American origin z Endotoxin levels< 0.1 EU/ml DD range - Serum Human plasma pool Part no. Volume (ml) € S4200-100 100 NC - Part no. Volume (ml) € S181B-500 500 NC - Part no. Volume (ml) € S4190-100 100 NC - Part no. Volume (ml) € S4180-100 100 NC - S4180-500 500 NC - Human serum HIV tested Human AB male serum HIV tested Animal plasma Human serum and plasma Part no. Designation Vol. (ml) € S0260-500 Bovine with sodium citrate 500 NC - S2600-500 Rabbit with EDTA 500 NC - Part no. Volume (ml) € S1860-100 100 NC - S1860-500 500 NC -
6 CELL CULTURE CELL CULTURE DD range - Lyophilised PH-7 BSA In 30% solution BSA (bovine serum albumin) DD range - BSA BSA Protease free Fatty acid free Quantity Part no. € Part no. € Part no. € 10 g P6154-10GR NC - - - - - 100 g P6154-100GR NC - P6155-100GR NC - P6156-100GR NC - 500 g P6154-500GR NC - P6155-500GR NC - - - Part no. Designation Vol. (ml) € A0296-100 BSA 30 % 100 NC - DMEM with phenol red GLASCOW MEM BHK21 HAM’S F10 HAM'S F12 LEIBOVITZ L15 McCOY’S 5A IMDM (Dulbecco's modified Iscove medium) with phenol red DMEM-HAM'S F12 with phenol red BME BASAL MEDIUM EAGLE z High glucose 4.5 g/l z Low glucose 1 g/l L-Glutamine HEPES NaHCO3 Sodium pyruvate Part no. Volume € Solution - 25 mM √ - L0100-500 500 ml NC - - - √ - L0101-500 500 ml NC - √ - √ - L0102-500 500 ml NC - Stable - √ √ L0103-500 500 ml NC - √ - √ √ L0104-500 500 ml NC - - - √ √ L0106-500 500 ml NC - Stable 25 mM √ - L0107-500 500 ml NC - Powder √ - - √ P0102-N1L 1 l NC - √ - - - P0103-N1L 1 l NC - L-Glutamine HEPES NaHCO3 Sodium pyruvate Part no. Volume € Solution - - √ √ L0090-500 500 ml NC - - - √ √ L0091-500* 500 ml NC - Stable 15 mM √ √ L0092-500 500 ml NC - √ 15 mM √ √ L0093-500 500 ml NC - - 15 mM √ √ L0094-500 500 ml NC - √ 25 mM √ √ L0095-500 500 ml NC - - 25 mM √ √ L0096-500 500 ml NC - Powder √ 15 mM - √ P0095-N1L 1 l NC - Earle's Salt Hank’s Salt L-Glutamine HEPES NaHCO3 Part no. Volume € Solution with phenol red √ - - - √ L0042-500 500 ml NC - L-Glutamine HEPES NaHCO3 Sodium pyruvate Part no. Volume € Solution √ - √ √ L0060-500 500 ml NC - - - √ √ L0064-500 500 ml NC - √ 25mM √ √ L0065-500 500 ml NC - Stable - √ √ L0066-500 500 ml NC - Powder √ - - √ P0061-N1L 1 l NC - L-Glutamine HEPES NaHCO3 Part no. Volume € Solution √ 25 mM √ L0190-500 500 ml NC - Stable 25 mM √ L0191-500 500 ml NC - - - √ L0192-500 500 ml NC - Powder √ 25 mM - P0191-N1L 1 l NC - L-Glutamine Part no. Volume € Powdered - without tryptose phosphate broth √ P0120-N1L 1 l NC - L-Glutamine HEPES NaHCO3 Part no. Volume € Solution with phenol red √ - √ L0140-500 500 ml NC - - - √ L0145-500 500 ml NC - Powder √ - - P0146-N1L 1 l NC - L-Glutamine NaHCO3 Part no. Volume € Solution with phenol red √ √ L0135-500 500 ml NC - - √ L0136-500 500 ml NC - Powder √ - P0134-N1L 1 l NC - * Glucose free DD range - Culture media Part no. Volume € In solution without L-glutamine with phenol red L0300-500 500 ml NC - Powder with L-Glutamine P0350-N1L 1 l NC - Part no. Volume € Solution without L-Glutamine L0210-500 500 ml NC - Powder with L-Glutamine, without NaHCO3 P0390-N1L 1 l NC - Tip Powder also available in 10 l and 50 l
7 Prices are indicative and excluding tax. Visit Dutscher.com CELL CULTURE CELL CULTURE M199 with phenol red MEM modified ALPHA MEM (Minimum Essential Medium) RMPI 1640 DD range - Culture media L-Glutamine Earle's Salt Hank’s Salt NEAA HEPES NaHCO3 Part no. Volume € Solution with phenol red √ √ - - - √ L0415-500 500 ml NC - Stable √ - - - √ L0416-500 500 ml NC - - √ - √ - √ L0430-500 500 ml NC - - √ - - - √ L0440-500 500 ml NC - √ √ - - 25 mM √ L0444-500 500 ml NC - - - √ - 25 mM √ L0470-500 500 ml NC - Powder √ √ - √ - - P0450-N1L 1 l NC - √ √ - - - - P0451-N1L 1 l NC - √ - √ √ - - P0515-N1L 1 l NC - L-Glutamine NaHCO3 Part no. Volume € Solution without ribonucleosides and deoxyribonucleosides √ √ L0475-500 500 ml NC - - √ L0476-500 500 ml NC - Powder with Earle’s salt √ - P0440-N1L 1 l NC - L-Glutamine Earle's Salt Hank’s Salt NaHCO3 HEPES Part no. Volume € Solution √ - √ √ - L0330-500 500 ml NC - √ √ - 1.25 g/l - L0355-500 500 ml NC - - √ - √ - L0356-500 500 ml NC - Stable √ - √ √ L0361-500 500 ml NC - Powder - - √ - - P0410-N1L 1 l NC - √ √ - - - P0420-N1L 1 l NC - √ √ - - 25 mM P0425-N1L 1 l NC - L-Glutamine HEPES Phenol red NaHCO3 Part no. Volume € Solution √ - √ √ L0500-100 100 ml NC - √ - √ √ L0500-500 500 ml NC - - - √ √ L0501-100 100 ml NC - - - √ √ L0501-500 500 ml NC - Stable - √ √ L0498-500 500 ml NC - √ 25 mM √ √ L0495-500 500 ml NC - Stable 25 mM √ √ L0496-500 500 ml NC - - 25 mM √ √ L0490-500 500 ml NC - - - √ √ L0503-500* 500 ml NC - - - - √ L0505-500 500 ml NC - Powder √ - √ - P0860-N1L 1 l NC - - - √ - P0870-N1L 1 l NC - √ - - - P0880-N1L 1 l NC - - - - - P0871-N1L 1 l NC - √ 25 mM - - P0876-N1L 1 l NC - √ - - - P0883-N1L 1 l NC - * without folic acid. DD range Schneider medium for drosophila Dutscher Range Schneider drosophila medium Part no. Vol. € L0207-500 500 ml NC -
8 CELL CULTURE CELL CULTURE DPBS EBSS - Earle's balanced salt solution, phenol red HBSS - Hank's balanced salt solution HEPES PBS without calcium, without magnesium Part no. Volume € DPBS without calcium, without magnesium L0615-100 100 ml NC - L0615-500 500 ml NC - L0615-1000 1 l NC - L0615-C10LS 10 l NC - DPBS 10 x without calcium, without magnesium X0515-500 500 ml NC - X0515-100 100 ml NC - X0515-C10LS 10 l NC - DPBS, 10 x with calcium, with magnesium X0520-500 500 ml NC - DPBS powder without calcium, without magnesium P0750-N1L 1 l NC - P0750-N10L 10 l NC - Part no. Designation € L0640-500 Sodium chloride saline solution 0.85% - 500 ml NC - L0643-100 Potassium chloride 0.075 M - 100 ml NC - L0643-500 Potassium chloride 0.075 M - 500 ml NC - P2035-500GR Potassium Chloride - 500 g NC - L0680-100 Sodium bicarbonate 7.5% - 100 ml NC - L0680-500 Sodium bicarbonate 7.5% - 500 ml NC - P2060-500GR Sodium bicarbonate, cell culture tested - 500 g NC - Other salts and buffer solutions DD range - Buffered saline solutions z Sterile, filtered z pH 7.4 -/+ 0.3 z No potassium chloride z Endotoxins < 1 EU/ml Part no. Designation Volume € L0601-500 Without calcium without magnesium 500 ml NC - EBSS 10x without sodium bicarbonate X0112-500 With calcium with magnesium 500 ml NC - X0113-500 Without calcium without magnesium 500 ml NC - Part no. Designation € L0180-100 HEPES 1M Buffer - 100 ml NC - L0180-500 HEPES 1M Buffer - 500 ml NC - Part no. Volume € L0616-500 500 ml NC - HBSS 10X X0507-500 - - - - 500 ml NC - X0509-500 √ √ - √ 500 ml NC - X0510-500 - - √ - 500 ml NC - X0513-500 - - - √ 500 ml NC - Dutscher Range Supplements Reagents Part no. Vol. (ml) € Colcemid 10 µg/ml L0040-010 10 NC - L0040-020 20 NC - L0040-050 50 NC - Sodium Pyruvate - 100 mM L0642-100 100 NC - L0642-500 500 NC - Dutscher Range Culture reagents: glutamine, amino acids Reagents Part no. Vol. (ml) € GLUTAMINE - 200 Mm X0550-100 100 NC - GLUTAMINE Stable - 200 mM X0551-100 100 NC - Vitamins MEM 100 x with glutamine X0556-100 100 NC - MEM NEAA 100 X without glutamine X0557-100 100 NC - Part no. Calcium Magnesium Sodium bicarbonate Phenol red Volume (ml) € L0605-500 - - - - 500 ml NC - L0606-500 √ √ √ √ 500 ml NC - L0607-500 - - √ - 500 ml NC - L0611-500 - - √ √ 500 ml NC - L0612-500 √ √ √ - 500 ml NC -
9 Prices are indicative and excluding tax. Visit Dutscher.com CELL CULTURE CELL CULTURE Dutscher Designation Concentration Volume Part no. € Amphotericin b (Fungizone) 250 µg/ml 50 ml L0009-050 NC - 100 ml L0009-100 NC - Antiobiotic-Antimycotic 100X 20 ml L0010-020 NC - 100 ml L0010-100 NC - Blasticidin S Hcl - 50 ml - - G 418 sulphate 50 mg/ml 20 ml L0015-020 NC - 100 ml L0015-100 NC - Gentamycin Sulphate 10 mg/ml 10 ml L0011-010 NC - 50 ml - - 100 ml L0011-100 NC - 50 mg/ml 10 ml L0012-010 NC - 50 ml - - 100 ml L0012-100 NC - Hygromycin B 50 mg/ml 20 ml - - 100 ml - - Kanamycin Sulphate 5 mg/ml 50 ml - - 100 ml - - 10 mg/ml 50 ml - - 50 mg/ml 50 ml - - 100 ml - - Antibiotic mixture in 8.5 Nacl - 50 ml - - - 100 ml - - Amphotericin 25 µg/ml - - - Penicillin 10,000 µl/ml - - - Streptomycin 100,000 µg/ml - - - Nanomycopulitine concentrate 20 X 10 ml L-X16-010 NC - 100 ml L-X16-100 NC - Neomycin Sulphate 10 mg/ml 50 ml - - 100 ml - - Nyastatin solution 10,000 U/ml 100 ml - - Penicillin-Streptomycin 100 ml L0018-100 NC - Penicillin/Streptomycin 5000 µl/ml - 5000 µg/ml 50 X 100 ml - - Penicillin/ Streptomycin 10000 U - 10 mg/ml 100 X 20 ml L0022-020 NC - 50 ml - - 100 ml L0022-100 NC - Dutscher Designation Volume Part no. € Amphotericin b (Fungizone) 25 mg - - 50 mg - - 100 mg - - G 418 sulphate 1 g P0017-1GR NC - 5 g P0017-5GR NC - Gentamycin Sulphate 1 g P4020-1GR NC - 5 g P4020-5GR NC - Hygromycin B 50 mg - - Kanamycin 10 g - - Penicillin G sodium salt 1 million units P0018-1MU NC - Penicillin G potassium salt 25 g - - Puromycin dihydrochlorin 0.1 g - - Streptomycin Sulphate 50 g - - Antibiotics solution Antibiotic powder z Mycoplasma PCR and electrophoresis detection z Ready to use z Sensitive and specific detection z Product shelf life 36 months MYCOKIT BOX z Non-toxic, non-volatile z Compatible with stainless steel Biogard A disinfectant for CO2 incubator water tank Mycoplasma prevention: incubator and surface Part no. Volume € K1000 20 tests NC - Part no. Designation Volume € 500177 Biogard A disinfectant for waterbaths and CO2incubators 100 ml NC - Part no. Designation Phenol red Vol. (ml) € L0909-100 Trypsin 0.25% in PBS √ 100 NC - L0910-100 Trypsin 0.25% in PBS 100 NC - L0930-100 Trypsin-EDTA 1X in PBS √ 100 NC - L0930-500 Trypsin-EDTA 1X in PBS √ 500 NC - L0931-100 Trypsin 0.25% - EDTA in HBSS √ 100 NC - L0932-100 Trypsin 0.25% - EDTA 0.02 % in HBSS √ 100 NC - X0915-100 Trypsin 2.5% in PBS 100 NC - L0940-100 Trypsin-EDTA 1X in PBS 100 NC - X0930-100 Trypsin -EDTA 10X 100 NC - X0920-100 Trypsin 2.5% in HBSS 100 NC - Trypsin calcium free magnesium free - solution Trypsin - powder Versene Accutase z Alternative to trypsin z Detachment and dissociation of adherent cells, without mammalian compounds DD Range Part no. Designation Weight € P5957-100GR Trypsin 1:250 powder (porcine) 100 g NC - Part no. Designation € L0630-100 Versene - 100 ml NC - Part no. Volume Storage T° € L0950100 100 ml -20 °C NC - Sterile, filtered, non-pyrogenic cell culture water. DD Range Part no. Vol. (ml) € L0970-100 100 NC - L0970-500 500 NC - L0970-1000 1000 NC - z Stimulates proliferation of lymphocytes in culture z Also used to separate leukocytes from whole blood Lymphocyte separation media PHA-M (Phytohaemagglutin) Part no. Designation Vol. (ml) € L0560-100 Lymphocyte separation medium 100 NC - L0560-500 Lymphocyte separation medium 500 NC - Part no. Designation Vol. € L3010-005 Phytohaemagglutinin liquid 5 ml NC -
10 MOLECULAR BIOLOGY Associated consumables MagnetaPure automated nucleic acid purification system Model MagnetaPure 32 Plus MagnetaPure 96 Number of samples 1 to 32 1 to 96 Sample volume per well 30 µl to 1 ml 50 µl to 1 ml Heating temperature Ambient at 120 °C Ambient at 120 °C Screen 7'' touchscreen 7'' touchscreen Storage capacity Up to 500 programmes Up to 100 programmes Extraction steps Lysis, retention, washing, elution Lysis, retention, washing, elution Connection 4 USB ports and ethernet 4 USB ports and ethernet Sterilization UV lamp UV lamp Barcode scanner No No Max. supply voltage (W) 450 450 Dimensions L x D x H (mm) 42 x 41 x 43 56 x 62 x 50 Weight (kg) 20 54 Part no. 255744 255745 € NC - NC - z Automated systems for the purification of DNA, RNA, proteins and cells from a wide variety of samples such as blood, bacteria, tissue, body fluids and plants z Magnetic separation technology for high throughput and high quality extraction without cross-contamination between samples or reagent transfers z Extraction of up to 96 samples simultaneously z Reaction volume from 30 µl to 1 ml z Fast extraction cycle: 20 to 60 minutes z Intuitive touch screen z Heating function for optimal lysis and elution z UV lamp for decontamination of the automat z Ready-to-use programs for a wide range of protocols 255745 255744 Part no. Designation Units/ box €/ box MagnetaPure 32 Plus 531557B Deepwell 96-well plate 50 NC - 531565B Comb 8 teeth 200 NC - MagnetaPure 96 531557B Deepwell 96-well plate 50 NC - 531556B Comb 96 teeth 100 NC - 531558B 96-well elution plates 100 NC -
11 Prices are indicative and excluding tax. Visit Dutscher.com z Injection moulded, durable, leak-proof tank designed to minimise the volume of buffer required z Each system is supplied with: an electrophoresis tank, a safety cover, one or two (DUO model) or three (TRIO model) casting trays, two combs, deposition guides and the casting walls to transform the tray into a casting system z Many optional combs z 4 sizes available: z ClearSub S: for 7 cm wide gels (up to 64 samples) z ClearSub M: for 10 cm wide gels (up to 100 samples) z ClearSub L: for 15 cm wide gels (up to 210 samples) z ClearSub XL: for 20 cm wide gels (up to 450 samples) ClearSub horizontal electrophoresis cells z Gels of size 7 x 7 and/or 7 x 10 cm z Gels in sizes 10 x 7 cm and/or 10 x 10 cm ClearSub S7 ClearSub S10 ClearSub SDUO Maximum number of samples 32 64 32 ; 64 Gel size / number of trays supplied 7 x 7 cm / 1 7 x 10 cm / 1 7 x 7 cm /1 ; 7 x 10 cm / 1 Combs supplied 2 combs with 8 teeth, thickness 1 mm Max. number of combs per tray 2 4 2 ; 4 Buffer volume (ml) 225 Typical migration condition 80 V, 45 - 60 minutes System dimensions (cm) 21 x 9 x 9 Max. electric power (W) 10 Part no. 257281 257282 257283 € NC - NC - NC - ClearSub M7 ClearSub M10 ClearSub MDUO Maximum number of samples 50 100 50 ; 100 Gel size / number of trays supplied 10 x 7 cm / 1 10 x 10 cm / 1 10 x 7 cm / 1 ; 10 x 10 cm / 1 Combs supplied 2 combs with 16 teeth, thickness 1 mm Max. number of combs per tray 2 4 2 ; 4 Buffer volume (ml) 300 Typical migration condition 90 V, 45-60 minutes System dimensions (cm) 22 x 12.5 x 9 Max. electric power (W) 20 Part no. 257284 257285 257286 € NC - NC - NC - ClearSub S System ClearSub M System Combs for ClearSub S Combs for ClearSub M Tooth thickness: 0.75 mm Tooth thickness: 1 mm Tooth thickness: 1.5 mm Tooth thickness: 2 mm Number of teeth Sample volume (µl) Part no. € Sample volume (µl) Part no. € Sample volume (µl) Part no. € Sample volume (µl) Part no. € 1 152 257323 NC - 203 257324 NC - 304 257325 NC - 405 257329 NC - 2 68 257343 NC - 90 257344 NC - 135 257345 NC - 180 257346 NC - 4 36 257347 NC - 48 257348 NC - 72 257349 NC - 96 257350 NC - 8 19 257326 NC - 25 257330 NC - 37 257334 NC - 50 257338 NC - 10 14 257327 NC - 18 257331 NC - 27 257335 NC - 36 257339 NC - 12 10 257332 NC - 14 257336 NC - 20 257340 NC - 27 257342 NC - 16 7 257328 NC - 10 257333 NC - 15 257337 NC - 20 257341 NC - Tooth thickness: 0.75 mm Tooth thickness: 1 mm Tooth thickness: 1.5 mm Tooth thickness: 2 mm Number of teeth Sample volume (µl) Part no. € Sample volume (µl) Part no. € Sample volume (µl) Part no. € Sample volume (µl) Part no. € 1 270 257351 NC - 360 257352 NC - 540 257353 NC - 720 257354 NC - 2 118 257364 NC - 158 257365 NC - 236 257371 NC - 315 257372 NC - 4 57 257378 NC - 77 257379 NC - 115 257380 NC - 153 257381 NC - 8 30 257355 NC - 41 257359 NC - 61 257366 NC - 81 257373 NC - 10 20 257356* NC - 27 257360* NC - 41 257367* NC - 54 257374* NC - 12 17 257357 NC - 23 257361 NC - 34 257368 NC - 45 257375 NC - 16 12 257358 NC - 16 257362 NC - 24 257369 NC - 32 257376 NC - 20 10 257407 NC - 14 257363 NC - 20 257370 NC - 27 257377 NC - * Compatible with multichannel micropipettes. Dutscher.com more products MOLECULAR BIOLOGY
12 MOLECULAR BIOLOGY z Gels of size 15 x 7 and/or 15 x 10 and/or 15 x 15 cm z Gels of size 20 x 10 cm and/or 20 x 20 cm or 20 x 15 cm, or 20 x 25 cm ClearSub L10 ClearSub L15 ClearSub LTRIO Maximum number of samples 140 210 70 ; 140 ; 210 Gel size / number of trays supplied 15 x 10 cm / 1 15 x 15 cm / 1 15 x 7 cm / 1 ; 15 x 10 cm / 1 ; 15 x 15 cm / 1 Combs supplied 2 combs with 20 teeth, thickness 1 mm Max. number of combs per tray 4 6 2 ; 4 ; 6 Buffer volume (ml) 500 Typical migration condition 90-150 V, 60 - 90 minutes System dimensions (cm) 26.5 x 17.5 x 9 Max. electric power (W) 40 Part no. 257287 257288 257289 € NC - NC - NC - ClearSub XL10 ClearSub XL15 ClearSub XL20 ClearSub XL25 ClearSub XLDUO Maximum number of samples 200 350 450 550 200 ; 450 Gel size / number of trays supplied 20 x 10 cm /1 20 x 15 cm/1 20 x 20 cm/1 20 x 25 cm/1 20 x 10/1; 20 x 20/1 Combs supplied 2 combs with 20 teeth, thickness 1 mm Max. number of combs per tray 4 7 9 11 4 ; 9 Buffer volume (ml) 1200 Typical migration condition 100-150 V, 60-90 minutes System dimensions (cm) 39.5 x 23 x 9 Max. electric power (W) 50 Part no. 257290 257291 257292 257293 257294 € NC - NC - NC - NC - NC - ClearSub L System ClearSub XL System Combs for ClearSub L Combs for ClearSub XL Tooth thickness: 0.75 mm Tooth thickness: 1 mm Tooth thickness: 1.5 mm Tooth thickness: 2 mm Number of teeth Sample volume (µl) Part no. € Sample volume (µl) Part no. € Sample volume (µl) Part no. € Sample volume (µl) Part no. € 1 371 257384 NC - 495 257385 NC - 743 257386 NC - 990 257387 NC - 2 169 257391 NC - 225 257393 NC - 338 257394 NC - 450 257395 NC - 4 91 257430 NC - 122 257431 NC - 182 257432 NC - 243 257433 NC - 10 34 257396 NC - 45 257403 NC - 68 257412 NC - 90 257423 NC - 10 22 257397* NC - 29 257404* NC - 44 257417* NC - 59 257424* NC - 12 30 257398 NC - 41 257405 NC - 61 257418 NC - 81 257425 NC - 14 22 257399* NC - 29 257406* NC - 44 257419* NC - 59 257426* NC - 16 20 257388* NC - 27 257389* NC - 41 257390* NC - 54 257392* NC - 20 16 257400 NC - 21 257408 NC - 32 257420 NC - 43 257427 NC - 28 8 257401* NC - 11 257409* NC - 17 257421* NC - 23 257428* NC - 35 7 257402 NC - 10 257410 NC - 15 257422 NC - 20 257429 NC - Tooth thickness: 0.75 mm Tooth thickness: 1 mm Tooth thickness: 1.5 mm Tooth thickness: 2 mm Number of teeth Sample volume (µl) Part no. € Sample volume (µl) Part no. € Sample volume (µl) Part no. € Sample volume (µl) Part no. € 1 506 257436 NC - 675 257437 NC - 1013 257435 NC - 1350 257438 NC - 2 236 257439 NC - 315 257440 NC - 473 257441 NC - 630 257442 NC - 4 115 257457 NC - 153 257465 NC - 230 257466 NC - 306 257467 NC - 10 54 257443 NC - 72 257450 NC - 108 257458 NC - 144 257468 NC - 16 30 257444 NC - 41 257451 NC - 61 257459 NC - 81 257469 NC - 20 20 257445* NC - 27 257452* NC - 41 257460* NC - 54 257470* NC - 25 16 257446 NC - 21 257453 NC - 32 257461 NC - 42 257471 NC - 30 13 257447 NC - 17 257454 NC - 26 257462 NC - 34 257472 NC - 36 11 257448 NC - 14 257455 NC - 22 257463 NC - 29 257473 NC - 40 8 257449* NC - 11 257456* NC - 17 257464* NC - 23 257474* NC - * Compatible with multichannel micropipettes. * Compatible with multichannel micropipettes.
13 Prices are indicative and excluding tax. Visit Dutscher.com MOLECULAR BIOLOGY z 2 ml dolphin nose microtubes with an insert containing a filtration membrane z Supplied assembled, caps closed z Working volume: 500 µl z Cellulose acetate or nylon membrane z Max centrifugal speed: 16000 g ClearSpin insert only without tube or membrane Part no. Designation Units/ bag €/ bag 007862CL ClearSpin insert only without tube or membrane 1000 NC - Membrane Cellulose acetate Nylon Porosity (µm) 0.22 0.45 0.22 Sterile No Yes Yes No Units/ bag 25 tubes + inserts 24 tubes + inserts 24 tubes + inserts 100 tubes + inserts Part no. 007856ACL 007857ACL 007859ACL 007860ACL €/ bag NC - NC - NC - NC - ClearSpin filtration microtubes Human DNA Pyro- gens PCR product purification kit - Exo PCR ClearUP z Cleaning of PCR products in one step z Quick and easy protocol (5 min) z Without the use of columns or magnetic balls z Removes residual primers, single-stranded DNA and inactivates excess dNTPs by dephosphorylation z Purified products for use in the preparation of NGS libraries Part no. Designation Number of purifications € 257635 Exo PCR ClearUP 100 NC - 257636 Exo PCR ClearUP 500 NC - 257637 Exo PCR ClearUP 5000 NC - ExtraClear Direct PCR ExtraClear Direct PCR kit ExtraClear Direct Hot Start PCR Kit z Kit for rapid DNA extraction (8 minutes) followed by PCR amplification and gel deposition z Extraction of DNA from various samples (mammalian tissues, leaves, feathers, fish fins, saliva,...) z Compatible with the use of automatic pipetting machines z Available in a classic version with Disco Taq enzyme, or in a Hot Start version with Hot Disco Taq enzyme z Contains ExtraClear non-toxic extraction solution and Disco Taq master mix 2X ready to load z Disco Taq master mix 2X ready to load is ready to use (addition of template, primers and water only) and contains a red dye for direct gel deposition z Supplied with ExtraClear non-toxic extraction solution and Hot Disco Taq master mix 2X ready to load z Hot Disco Taq master mix 2X ready to load is ready to use (addition of template, primers and water only) and contains a red dye for direct gel deposition z Hot Disco Taq is inactivated at room temperature, activation at 95°C for 15 min Part no. Designation Number of purifications € 257732 ExtraClear Direct PCR kit 100 NC - 257733 ExtraClear Direct PCR kit 500 NC - Part no. Designation Number of purifications € 257730 ExtraClear Direct Hot Start PCR Kit 100 NC - 257731 ExtraClear Direct Hot Start PCR Kit 500 NC - DNA extraction kit - ExtraCLEAR z DNA extraction from various samples (bacteria, tissue, plants, organs, bird feathers, saliva) z Quick and easy protocol (8 minutes) z Once the reagent has been mixed with the sample, it is simply incubated at 3 different temperatures (65°C 6 min, 98°C 2 min, 4°C) z Extraction carried out in a themocycler or heating block z Non-toxic reagent z Extracted DNA ready for PCR amplification Part no. Designation Number of purifications € 257747 ExtraCLEAR DNA extraction kit 100 NC - 257748 ExtraCLEAR DNA extraction kit 500 NC - Tip Find ExtraClear solutions for DNA extraction on page 235 and Disco Taq and Disco Taq Fever master mix 2X, ready to use on page 246
14 MOLECULAR BIOLOGY Disco taq DNA polymerase Disco taq 5 U/µl, with or without buffer and MgCl2 Disco taq and Disco taq Fever master mix 2X, ready to use z High throughput and reliable amplification for routine applications, screening or high-throughput screening z Amplification up to 5 kb, compatible with TA-cloning and dUTP incorporation z 5' -> 3' exonuclease activity and absence of 3'-> 5' exonuclease activity z Disco taq polymerase 5 U/µl, with or without 10X buffer and MgCl2 z Complete with dNTPs, the matrix, primers, water, 10X buffer and MgCl2 if not supplied z Several stamps available z Master mix ready to use, addition of matrix, primers and water only z The Disco taq 2X master mix contains Disco taq DNA polymerase, NH4+ buffer, 0.4 mM of each dNTP, MgCl2 at a final concentration of 1.5 or 2 mM, and stabilising agents z The Disco taq Fever 2X master mix is supplied with a Fever buffer, for amplifications of up to 4kb with increased specificity, even with difficult templates Available in a "ready to load" version, containing a loading buffer and a red dye to monitor migration: + Can be deposited directly after PCR on an agarose or SDS page gel + No interference with PCR, the products obtained can be used for downstream applications (sequencing, etc.) after purification on a column such as PureIT Exo PCR ClearUp Specificity Optimisation of MgCl2 concentration and required hybridisation temperature - Standard buffer (Tris-HCl pH 8.5, KCl, 15 mM MgCl2, 1% Tween 20) +++ Yes - Ammonium buffer (Tris-HCl pH 8.5, (NH4)2S04, 15 mM MgCl2, 1% Tween 20) + No - Combination buffer (Tris-HCl, pH 8.7, KCl, (NH4)2SO4, 15 mM MgCl2, 1% Tween 20) ++ Depending on the matrix and primers Designation Number of 50 µl reactions Without buffer and without MgCl 2 € 10X standard buffer and final 25 mM MgCl2 € 10X ammonium buffer and final 25 mM MgCl2 € 10X combined buffer and final mM MgCl2 € Part no. Part no. Part no. Part no. Disco taq 5 U/µl 500 257776 NC - 257604 NC - 257548 NC - 258332 NC - 1000 257777 NC - 257593 NC - 257545 NC - 257592 NC - 2500 257778 NC - 257597 NC - 257546 NC - 257596 NC - 5000 257779 NC - 257600 NC - 257547 NC - 257599 NC - Designation Number of 50 µl reactions Number of 20 µl reactions Final MgCl2 concentration: 1.5 mM € Final MgCl2 concentration: 2 mM € Part no. Part no. Disco taq master mix 2X 100 250 257553 NC - 257563 NC - 500 1250 257555 NC - 257565 NC - 2500 6250 257554 NC - 257564 NC - 5000 12500 257556 NC - 257566 NC - Disco taq Fever master mix 2X 100 250 257549 NC - - - 500 1250 257551 NC - - - 2500 6250 257550 NC - - - 5000 12500 257552 NC - - - Disco taq master mix 2X, ready to load 100 250 257557 NC - 257567 NC - 500 1250 257561 NC - 257571 NC - 2500 6250 257560 NC - 257570 NC - 5000 12500 257562 NC - 257572 NC - Buffers available separately on page 257 Samples available, contact us
15 Prices are indicative and excluding tax. Visit Dutscher.com MOLECULAR BIOLOGY Disco Taq without glycerol z Disco taq 5 U/µl or 50 U/µl with glycerol-free storage buffer, with or without 10X buffer and 25 mM MgCl2 z For applications involving a freeze-drying step, or requiring precise pipetting of small volumes (manual or via a pipetting machine) z 1U allows an average of 500 reactions to be carried out z Several stamps available Specificity Optimisation of MgCl2 concentration and required hybridisation temperature - Standard buffer (Tris-HCl pH 8.5, KCl, 15 mM MgCl2, 1% Tween 20) +++ Yes - Ammonium buffer (Tris-HCl pH 8.5, (NH4)2S04, 15 mM MgCl2, 1% Tween 20) + No Designation Number of units (U) Without buffer and without MgCl2 € 10X standard buffer and final 25 mM MgCl2 € 10X ammonium buffer and final 25 mM MgCl2 € Part no. Part no. Part no. Disco taq 5 U/µl, glycerol-free 500 257590 NC - 257587 NC - 257585 NC - 1000 257588 NC - 257578 NC - 257579 NC - 2500 257780 NC - 257581 NC - 257781 NC - 5000 257589 NC - 257584 NC - 257582 NC - Disco taq 50U/µl, glycerol-free 25000 257575 NC - - - - - 250000 257574 NC - - - - - 2000000 257573 NC - - - - - Buffers available separately on page 257 Disco taq DNA polymerase (continued)
16 MOLECULAR BIOLOGY Hot Disco Taq DNA polymerase Hot Disco Taq 5U/µl, with or without buffer and MgCl2 Hot Disco Taq master mix 2X, ready to use z Inactivated at room temperature, activation at 95°C for 15 min z Possible incorporation of dUTP z High specificity and sensitivity for high performance z For low-level target detection, screening, mutliplexing, direct colony PCR, and qPCR applications z Hot Disco Taq polymerase 5 U/µl, with or without 10X buffer and MgCl2 z Complete with dNTPs, the matrix, primers, water, 10X buffer and MgCl2 if not supplied z Several stamps available z Master mix ready to use, addition of matrix, primers and water only z The 2X master mix contains Hot Disco Taq DNA polymerase, dNTPs, MgCl2 at a final concentration of 1.5 mM, and buffer 1 (KCI/(NH4)2 SO4 Tris) or buffer 2 ((NH4)2 SO4) Specificity Optimisation of MgCl2 concentration and required hybridisation temperature - Standard buffer (Tris-HCl pH 8.5, KCl, 15 mM MgCl2, 1% Tween 20) +++ Yes - Ammonium buffer (Tris-HCl pH 8.5, (NH4)2S04, 15 mM MgCl2, 1% Tween 20) + No - Combination buffer (Tris-HCl, pH 8.7, KCl, (NH4)2SO4, 15 mM MgCl2, 1% Tween 20) ++ Depending on the matrix and primers Designation Number of units / Number of 50 µl reactions Without buffer and without MgCl2 € 10X standard buffer and 25 mM MgCl2 € 10X ammonium buffer and 25 mM MgCl2 € 10X combined buffer and mM MgCl2 € Part no. Part no. Part no. Part no. Hot Disco Taq 5U/µl 500 257674 NC - - - 257670 NC - 257782 NC - 1000 257658 NC - 257661 NC - 257659 NC - 257660 NC - 2500 257662 NC - 257665 NC - 257663 NC - 257664 NC - 5000 257666 NC - 257669 NC - 257667 NC - 257668 NC - Designation Number of 50 µl reactions Number of 20 µl reactions Buffer 1 € Buffer 2 € Part no. Part no. Hot Disco Taq master mix 2X 100 250 257683 NC - 257695 NC - 500 1250 257685 NC - 257697 NC - 2500 6250 257684 NC - 257696 NC - 5000 12500 257686 NC - 257698 NC - Hot Disco Taq master mix 2X, Ready to load 100 250 257687 NC - 257691 NC - 500 1250 257689 NC - 257693 NC - 2500 6250 257688 NC - 257692 NC - 5000 12500 257690 NC - 257694 NC - Buffers available separately on page 257 Available in a "Ready to load" version, containing a loading buffer and a red dye to monitor migration: + Can be deposited directly after PCR, on agarose or SDS gel + No interference with PCR, the products obtained can be used for downstream applications (sequencing, etc.) after purification on a column such as PureIT Exo PCR ClearUp Samples available, contact us Hot Disco Taq GC master mix 2X, ready to use z Master mix ready to use, addition of matrix, primers and water only z For the amplification of GC-rich DNA sequences z Minimises the risk of artefacts due to possible aspecific polymerisation before the first denaturation step z The 2X master mix contains Hot Disco Taq DNA polymerase, optimised buffer, dNTPs, 3.0 mM MgCl2 and stabilising agents Designation Number of 50 µl reactions Number of 20 µl reactions Master mix 1 € Master mix 2 € Part no. Part no. Hot Disco Taq 5U/µl 100 250 257675 NC - 257679 NC - 500 1250 257677 NC - 257681 NC - 2500 6250 257676 NC - 257680 NC - 5000 12500 257678 NC - 257682 NC - Available with master mix I or II. : + Master mix I is suitable in most cases. If, however, the results obtained are not satisfactory, we recommend using master mix 2 + Contact us for samples to select the master mix that will optimise your results
17 Prices are indicative and excluding tax. Visit Dutscher.com MOLECULAR BIOLOGY Hot Disco Taq DNA polymerase (continued) Hot Disco Taq without glycerol, with or without buffer 10X Hot Disco Taq master mix (5X) lyophilised z Hot Disco Taq 5U/µl with glycerol-free storage buffer, with or without 10X buffer and 25 mM MgCl2 z For applications involving a freeze-drying step, or requiring precise pipetting of small volumes (manual or via a pipetting machine) or automated high-throughput screening z Several stamps available z Ready to use once reconstituted in the supplied buffer, adding only the matrix, primers and water z Stable for 12 months at room temperature: store and transport at room temperature z Amplification up to 4 kb z The reconstituted Hot Disco Taq master mix (5X) is composed of Hot Disco Taq DNA polymerase, dNTPs, NH4+ buffer and MgCl2 z Available in a "Ready to load" version, containing two dyes (orange G and xylene cyanol FF) and a loading buffer, for monitoring migration Designation Number of 25 µl reactions Part no. € Hot Disco Taq master mix 5X lyophilised 500 257701 NC - 1000 257699 NC - 2500 257700 NC - Hot Disco Taq master mix 5X lyophilised, ready to load 500 257704 NC - 1000 257702 NC - 2500 257703 NC - Specificity Optimisation of MgCl2 concentration and required hybridisation temperature - Standard buffer (Tris-HCl pH 8.5, KCl, 15 mM MgCl2, 1% Tween 20) +++ Yes - Ammonium buffer (Tris-HCl pH 8.5, (NH4)2S04, 15 mM MgCl2, 1% Tween 20) + No - Combination buffer (Tris-HCl, pH 8.7, KCl, (NH4)2SO4, 15 mM MgCl2, 1% Tween 20) ++ Depending on the matrix and primers Designation Number of units (U) Without buffer and without MgCl2 € 10X standard buffer and 25 mM MgCl2 € 10X ammonium buffer and 25 mM MgCl2 € Part no. Part no. Part no. Hot Disco taq 5U/µl, glycerol-free 500 257713 NC - 257725 NC - 257723 NC - 1000 257709 NC - 257716 NC - 257714 NC - 2500 257711 NC - 257719 NC - 257717 NC - 5000 257712 NC - 257722 NC - 257720 NC - Buffers available separately on page 257 Hot Disco Taq Multiplex master mix 2X, ready to use z Master mix 2X ready to use, addition of matrix, primers and water only z Taq inactivated at room temperature, activation at 95 °C for 15 min z Simultaneous amplification of several targets in a single tube z High specificity and sensitivity for high performance z Minimises the risk of artefacts due to possible aspecific polymerisation before the first denaturation step z Possible incorporation of dUTP z The 2X buffer contains Hot Disco Taq DNA polymerase, dNTPS, optimised buffer and 6 mM MgCl2 Designation Number of 50 µl reactions Number of 20 µl reactions Part no. € Hot Disco Taq Multiplex master mix 2X 100 250 257705 NC - 500 1250 257707 NC - 2500 6250 257706 NC - 5000 12500 257708 NC -
18 MOLECULAR BIOLOGY ClearLine deoxyribonucleotides (dNTPs), PCR grade water and enhancer Part no. Designation Concentration Packaging € Deoxyribonucleotides 257743 Mix of dNTPs, solutions of dATP, dCTP, dGTP and DTTp, 25 mM of each 100 mM 8 tubes of 0.5 ml NC - 257744 Mix of dNTPs, solutions of dATP, dCTP, dGTP and DTTp, 10 mM of each 40 mM 2 tubes of 0.5 ml NC - 257746 Set of dNTP, solution of dATP, dCTP, dGTP and DTTp, 100 µM, in separate tubes 100 mM 16 tubes of 0.25 ml NC - 257745 DNTP set, solution of dATP, dCTP, dGTP and DTTp, 25µM, in separate tubes 100 mM 4 tubes of 0.25 ml NC - MgCl2 257741 MgCl2 25 mM 25 mM 10 tubes of 1.5 ml NC - 257742 MgCl2 25 mM 25 mM 3 tubes of 1.5 ml NC - Enhancer PCR 257543 Betaine solution enhancer for complex matrices 5 M 5 tubes of 1 ml NC - PCR grade water 257605 PCR grade ultrapure water - 1 bottle of 1000 ml NC - 257606 PCR grade ultrapure water - 1 bottle of 500 ml NC - 257607 PCR grade ultrapure water - 6 tubes of 5 ml NC - Part no. Designation Concentration Packaging € 257757 10X standard buffer, 1.7mM MgCl2 for Hot Disco Taq and Disco Taq DNA polymerase 10X 3 tubes of 1.5 ml NC - 257749 10X ammonium buffer, 1.5 mM MgCl2 Hot Disco Taq and Disco Taq DNA polymerase 10X 3 tubes of 1.5 ml NC - 257750 10X combined buffer, 1.5 mMMgCl2 Hot Disco Taq and Disco Taq DNA polymerase 10X 3 tubes of 1.5 ml NC - 257755 GC-rich matrix buffer 1 4X Hot Disco Taq DNA polymerase 4X 10 tubes of 1.5 ml NC - 257756 GC-rich matrix buffer 2 4X Hot Disco Taq DNA polymerase 4X 10 tubes of 1.5 ml NC - Buffers for Disco Taq and Hot Disco Taq DNA polymerase Hi-fi Pop DNA polymerase 2 U/µl, with 5X buffer and MgCl2 z Hi-fi Pop DNA polymerase 2 U/µl, with 5X Hi-fi buffer and 25 mM MgCl2 z Amplification of up to 18kb of gDNA and 25kb of DNA λ z To be completed with dNTPs, matrix, primers and water Hi-fi Pop DNA polymerase z For applications that require high fidelity such as cloning, sequencing, SNP analysis and mutagenesis z 60 times more accurate than conventional Taq polymerase z Amplification of long, GC-rich DNA sequences z Complex matrices (very rich in GC, complex secondary structures, very long sequences, etc.) may require the addition of a 5M betaine enhancer (optional) z High elongation rate: 10 sec/kb z 3’ –› 5' exonuclease activity Hi-fi Pop DNA polymerase master mix 2X, ready to use z Master mix ready to use, addition of matrix, primers and water only z Amplification of up to 11 kb of gDNA z The 2X buffer contains Hi-fi Taq DNA polymerase, dNTPs, Hi-fi buffer and 3 mM MgCl2 for a final concentration of 1.5 mM Designation No. of units / no. of 50 µl reactions 5X Hi-fi buffer and 25 mM MgCl2 € Hi-fi Pop DNA polymerase 2 U/µl 100 257651 NC - 500 257653 NC - 2500 257650 NC - 5000 257652 NC - Designation Number of 50 µl reactions Number of 20 µl reactions Part no. € Hi-fi Pop DNA polymerase Master mix 2X 100 250 257654 NC - 500 1250 257656 NC - 2500 6250 257655 NC - 5000 12500 257657 NC -
19 Prices are indicative and excluding tax. Visit Dutscher.com MOLECULAR BIOLOGY Scale ladder Part no. 257739 257738 257740 € NC - NC - NC - Size of fragments From 100 to 3000 bp From 100 to 1000 bp From 100 to 500 bp No. of fragments 7 5 3 Double intensity fragments 700 pb 500 pb - Concentration 24 µg/ml 19 µg/ml 12 µg/ml Quantity / Volume 12 µg/500 µl 9.5 µg/500 µl 6 µg/500 µl Vol. to be used/wells 5 µl (100 lines/tube) Ready to use (including dyes for migration monitoring and glycerol) Yes Type of dye Bromophenol blue z Ready to use z For DNA and RNA on polyacrylamide or agarose gel Part no. Designation Dye for migration monitoring Position of dye in relation to samples (1% agarose gel) Concentration Packaging € 257752 Cyan charge buffer Xylene cyanol FF 800 - 1000 bp 5X 5 tubes of 1 ml NC - 257754 Red charge buffer Cresol red 500 - 800 bp 5X 5 tubes of 1 ml NC - 257751 Blue charge buffer Bromophenol blue 300 - 500 bp 5X 5 tubes of 1 ml NC - 257753 Orange charge buffer Orange G 50 - 150 bp 5X 5 tubes of 1 ml NC - Charge pad High sensitivity for small fragments, low background noise, dye to be added during gel preparation or following migration, 4 - 6 µl per 100 ml agarose. z For nucleic acid marking z Non-carcinogenic alternative to ethidium bromide z DMSO-free storage solution z Main excitation wavelength 490 nm / secondary 290 nm z Emission wavelength 530 nm Part no. Designation € 755110CL EmeralDye 1 ml NC - ClearLine® EmeralDye colourant Fast and easy to use. z 1 sachet makes 1 litre of 1X buffer z No pH adjustment required ClearLine® powder buffers for nucleic acid electrophoresis Part no. Designation Composition pH Units/ box €/ box 257081 Tris-AcetateEDTA (TAE) buffers 40 mM Tris, 20 mM acetic acid, 1 mM EDTA 8.2 - 8.4 100 NC -
20 MOLECULAR BIOLOGY z To annotate PVDF or nitrocellulose transfer membranes z Reacts with HRP substrates z Fast and sensitive z Compatible with X-ray film and CCD imagers Pre-coloured chemiluminescent marker for Western blotting Part no. Designation € 701100 Pre-stained chemiluminescent marker for Western blot NC - ClearLine® SolidFrame bi-component PCR plates z High quality plates for all PCR and qPCR applications z Rigid polycarbonate frame z Plate rigidity prevents deformation by temperature and the risk of evaporation due to loss of sealing with the film z Ideal for robot handling z Ultra-thin walled blank medical grade polypropylene wells for optimum temperature transfer z HS (high sensitivity) white well model for improved detection of low abundance targets Frame colour Well colour Skirt Style Compatibility Part no. € Standard profile (0.2 ml) Natural Natural Half-skirted ABI 5 257301 NC - Natural White Half-skirted ABI 257302 NC - Natural Natural Half-skirted Universal 3 257298 NC - Natural White Half-skirted Universal 257299 NC - Natural High sensitivity white Half-skirted Universal 257300 NC - Low profile (0.1 ml) White White Half-skirted Roche 6 257311 NC - White White, highly sensitive Half-skirted Roche 257312 NC - Natural Natural Half-skirted Roche 257296 NC - Natural White Half-skirted Roche 257297 NC - White Natural Skirted Standard 8 257308 NC - White White Skirted Standard 257309 NC - White White, highly sensitive Skirted Standard 257310 NC - Black Natural Skirted Standard 257303 NC - Black White Skirted Standard 257304 NC - Black White, highly sensitive Skirted Standard 257305 NC - Frame colour Well colour Skirt Compatibility Part no. € Black Natural Skirted 9 257306 NC - Black White Skirted 257307 NC - White White Skirted 10 257313 NC - 96-well plates 384-well plates Dutscher.com more info Human DNA Fast and easy to use. z 1 sachet makes 1 litre of 1X buffer z No pH adjustment required z Fast running buffer for high voltage electrophoresis (300 - 350 V); allows rapid separation of fragments of less than 5 kb (a conventional 30 min cycle can be completed in only 10 min) ClearLine® powder buffers for nucleic acid electrophoresis Part no. Designation Composition pH Units/box €/ box 257083 Fast running buffer - 8.1-8.5 100 NC -
21 Prices are indicative and excluding tax. Visit Dutscher.com MOLECULAR BIOLOGY ClearLine® PCR plates FastGene ClearLine® white semi-skirted low profile 96-well PCR plate Part no. Compatibility Skirt Work volume Format Other features Units/ box €/ box 257047 1 Without skirt 200 µl Universal Flange around each well for easy sealing 100 NC - 257048 8 Skirted 100 µl Universal For low profile block, CFX96 compatible 100 NC - 257049 4 Halfskirted 200 µl Universal / ABI With plate flanges for alignment with purification plates 100 NC - 257050 4 Halfskirted 200 µl Universal /ABI With edges in the 4 corners of the plate 100 NC - z 96-well plate z Natural colour z Alphanumeric index printed in black z Ultra-thin, uniform wall for excellent heat transfer and optimum results z Produced from ultrapur polypropylene z Plate compatible with LigthCycler, Biorad CFX96 and Abi stepOne Plus z Total volume 0.2 ml, maximum working volume 0.1 ml z Alphanumeric code Human DNA Human DNA Pyro- gens Pyro- gens PCR inhibitor Part no. Designation Compatibility € for 50 257783 FastGene white semi-skirted low profile 96-well PCR plate See on Dutscher.com NC -
22 MOLECULAR BIOLOGY APPLICATION: PCR qPCR (optically clear) Short-term storage / incubation / ELISA Long-term storage Cell culture and microbiology HPLC Fluorescence / luminescence Part no. Packaging Model Highlights 760212 100 sheets AlumaSeal® II™ Does not withdraw into itself • • 760213 50 sheets AlumaSeal® II™ Does not withdraw into itself • • 760231 100 sheets AlumaSeal® 96™ Reinforced sealing for 96-well plates • • 760232 100 sheets AlumaSeal® 384™ Reinforced sealing for 384-well plates • • 760206 100 sheets ThermalSeal® Easy to remove • • 760207 100 sheets ThermalSeal® Easy to remove • • 765267 100 sheets eXTReme™ Seal Superior adhesion minimising evaporation • 765276 100 sheets eXTReme™ Seal Large Superior adhesion minimising evaporation • 760229 100 sheets ThermalSeal® A™ Superior adhesion minimising evaporation • 765264 100 sheets ThermalSeal® 2™ Superior adhesion minimising evaporation • 765269 400 sheets EZcap™ 1 x 8 PCR FilmStrips™ Scored sheet per column of 8 wells • 760248 200 sheets ThermalSeal® 2x8 MiniStrips® Scored sheet in 2 columns of 8 wells • 765282 100 sheets FoilSeal™ Opaque • 765281 50 sheets FoilSeal™ Opaque • 765268 300 sheets EZcap™ 1 x 8 PCR FoilSeal™ Scored sheet per column of 8 wells • 765270 1 roll FoilSeal™ Excellent chemical resistance • 765246 100 sheets ThermalSeal® RTS™ Silicone adhesive is released only when pressure is applied for a perfect adhesion without interference with the sample • • • 765265 50 sheets ThermalSeal® RTS™ Silicone adhesive is released only when pressure is applied for a perfect adhesion without interference with the sample • • • 760236 100 sheets ThermalSeal® RT2RR™ High optical quality films with excellent adhesion • 760208 100 sheets ThermalSeal® RT2™ High optical quality films with excellent adhesion • 765272 1 roll SealPlate® Minimises evaporation, available with colour spotlight • 765273 400 sheets 1 x 8 MiniStrips™ Scored sheet per column of 8 wells • 765274 400 sheets 1 x 8 MiniStrips™ Scored sheet per column of 8 wells • 760202 200 sheets 2 x 8 MiniStrips™ Scored sheet in 2 columns of 8 wells • 760203 200 sheets 2 x 8 MiniStrips™ Scored sheet in 2 columns of 8 wells • 760227 50 sheets SealPlate® Minimises evaporation, available with colour spotlight (assortments) • 760226 100 sheets SealPlate® Minimises evaporation, available with colour spotlight (blue) • 760222 100 sheets SealPlate® Minimises evaporation, available with colour spotlight (green) •
23 MOLECULAR BIOLOGY SPECIFICATIONS ADHESIVE CHARACTERISTICS COMPATIBILITIES Dimensions without tab (mm) Sterile DNase RNase free Chemical resistance Drillable Peelable Transparent Opaque Film / adhesive material Operating temperature Film thickness / adhesive (µm) Type of plate Raised rim • • • • Aluminium / Acrylic -80 °C to +120 °C 35,6 / 27,9 Skirt and half-skirt Without raised rim 125.4 x 82.6 • • • • • Aluminium / Acrylic -80 °C to +120 °C 35,6 / 27,9 Skirt and half-skirt Without raised rim 125.4 x 82.6 • • • Aluminium / Acrylic -80 °C to +120 °C 38,1 / 37,6 All types of skirts With raised rim 117.5 x 77.8 • • • Aluminium / Acrylic -80 °C to +120 °C 38,1 / 37,6 Skirt and half-skirt Without raised rim 123.8 x 82.6 • • • • Polypropylene / Acrylic -40 °C to +120 °C 50,8 / 24,1 Skirt and half-skirt Without raised rim 123.1 x 79.4 • • • • • Polypropylene / Acrylic -40 °C to +120 °C 50,8 / 24,1 Skirt and half-skirt Without raised rim 123.1 x 79.4 • • • Polyester / Acrylic -20 °C to +120 °C 50,8 / 41,9 All types of skirts With or without raised rim 118.1 x 78.3 • • • Polyester / Acrylic -20 °C to +120 °C 50,8 / 41,9 Skirt and half-skirt Without raised rim 121.9 x 81.5 • • Polyester / Acrylic -40 °C to +120 °C 50,8 / 41,9 All types of skirts With raised rim 118.1 x 78.3 • • Polyester / Acrylic -40 °C to +120 °C 50,8 / 41,9 Skirt and half-skirt Without raised rim 125.1 x 79.4 • • Polypropylene / Acrylic -20 °C to +120 °C 50,8 / 41,9 Skirt and half-skirt Without raised rim 73.3 x 10.1 • • • Polypropylene / Acrylic -40 °C to +120 °C 50,8 / 26,7 Skirt and half-skirt With raised rim 76.2 x 19.1 • • • • Aluminium / Acrylic -20 °C to +120 °C 50,8 / 36,8 All types of skirts With or without raised rim 118.1 x 78.3 • • • • • Aluminium / Acrylic -20 °C to +120 °C 50,8 / 36,8 All types of skirts With or without raised rim 118.1 x 78.3 • • • • Aluminium / Acrylic -20 °C to +120 °C 50,8 / 36,8 Skirt and half-skirt Without raised rim 81.2 x 10.1 • • • • Aluminium / Acrylic -20 °C to +120 °C 50,8 / 36,8 All types of skirts With or without raised rim 117.1 x 78.3 • • Polyolefin / Silicone -80°C to +105°C 50,8 / 50,8 All types of skirts With or without raised rim 116.2 x 78.3 • • • Polyolefin / Silicone -80°C to +105°C 50,8 / 50,8 All types of skirts With or without raised rim 116.2 x 78.3 • Polyester / Acrylic -40 °C to +120 °C 50,8 / 29,2 All types of skirts With or without raised rim 118.1 x 77.8 • Polyester / Acrylic -40 °C to +120 °C 50,8 / 29,2 Skirt and half-skirt Without raised rim 121.9 x 79.4 • • Polyester / Acrylic -40 °C to +120 °C 50,8 / 26,7 Skirt and half-skirt Without raised rim 121.9 x 79.4 • • Polyester / Acrylic -40 °C to +120 °C 50,8 / 26,7 Skirt and half-skirt Without raised rim 82.6 x 9.5 • • • Polyester / Acrylic -40 °C to +120 °C 50,8 / 26,7 Skirt and half-skirt Without raised rim 82.6 x 9.5 • Polypropylene / Acrylic -40 °C to +120 °C 50,8 / 26,7 Skirt and half-skirt Without raised rim 82.6 x 19.1 • • Polypropylene / Acrylic -40 °C to +120 °C 50,8 / 26,7 Skirt and half-skirt Without raised rim 82.6 x 19.1 • • Polyester / Acrylic -40 °C to +120 °C 50,8 / 26,7 Skirt and half-skirt Without raised rim 125 x 79.4 • • Polyester / Acrylic -40 °C to +120 °C 50,8 / 26,7 Skirt and half-skirt Without raised rim 125 x 79.4 • • Polyester / Acrylic -40 °C to +120 °C 50,8 / 26,7 Skirt and half-skirt Without raised rim 125 x 79.4
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