14 MOLECULAR BIOLOGY Disco taq DNA polymerase Disco taq 5 U/µl, with or without buffer and MgCl2 Disco taq and Disco taq Fever master mix 2X, ready to use z High throughput and reliable amplification for routine applications, screening or high-throughput screening z Amplification up to 5 kb, compatible with TA-cloning and dUTP incorporation z 5' -> 3' exonuclease activity and absence of 3'-> 5' exonuclease activity z Disco taq polymerase 5 U/µl, with or without 10X buffer and MgCl2 z Complete with dNTPs, the matrix, primers, water, 10X buffer and MgCl2 if not supplied z Several stamps available z Master mix ready to use, addition of matrix, primers and water only z The Disco taq 2X master mix contains Disco taq DNA polymerase, NH4+ buffer, 0.4 mM of each dNTP, MgCl2 at a final concentration of 1.5 or 2 mM, and stabilising agents z The Disco taq Fever 2X master mix is supplied with a Fever buffer, for amplifications of up to 4kb with increased specificity, even with difficult templates Available in a "ready to load" version, containing a loading buffer and a red dye to monitor migration: + Can be deposited directly after PCR on an agarose or SDS page gel + No interference with PCR, the products obtained can be used for downstream applications (sequencing, etc.) after purification on a column such as PureIT Exo PCR ClearUp Specificity Optimisation of MgCl2 concentration and required hybridisation temperature - Standard buffer (Tris-HCl pH 8.5, KCl, 15 mM MgCl2, 1% Tween 20) +++ Yes - Ammonium buffer (Tris-HCl pH 8.5, (NH4)2S04, 15 mM MgCl2, 1% Tween 20) + No - Combination buffer (Tris-HCl, pH 8.7, KCl, (NH4)2SO4, 15 mM MgCl2, 1% Tween 20) ++ Depending on the matrix and primers Designation Number of 50 µl reactions Without buffer and without MgCl 2 € 10X standard buffer and final 25 mM MgCl2 € 10X ammonium buffer and final 25 mM MgCl2 € 10X combined buffer and final mM MgCl2 € Part no. Part no. Part no. Part no. Disco taq 5 U/µl 500 257776 NC - 257604 NC - 257548 NC - 258332 NC - 1000 257777 NC - 257593 NC - 257545 NC - 257592 NC - 2500 257778 NC - 257597 NC - 257546 NC - 257596 NC - 5000 257779 NC - 257600 NC - 257547 NC - 257599 NC - Designation Number of 50 µl reactions Number of 20 µl reactions Final MgCl2 concentration: 1.5 mM € Final MgCl2 concentration: 2 mM € Part no. Part no. Disco taq master mix 2X 100 250 257553 NC - 257563 NC - 500 1250 257555 NC - 257565 NC - 2500 6250 257554 NC - 257564 NC - 5000 12500 257556 NC - 257566 NC - Disco taq Fever master mix 2X 100 250 257549 NC - - - 500 1250 257551 NC - - - 2500 6250 257550 NC - - - 5000 12500 257552 NC - - - Disco taq master mix 2X, ready to load 100 250 257557 NC - 257567 NC - 500 1250 257561 NC - 257571 NC - 2500 6250 257560 NC - 257570 NC - 5000 12500 257562 NC - 257572 NC - Buffers available separately on page 257 Samples available, contact us
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