17 Prices are indicative and excluding tax. Visit Dutscher.com MOLECULAR BIOLOGY Hot Disco Taq DNA polymerase (continued) Hot Disco Taq without glycerol, with or without buffer 10X Hot Disco Taq master mix (5X) lyophilised z Hot Disco Taq 5U/µl with glycerol-free storage buffer, with or without 10X buffer and 25 mM MgCl2 z For applications involving a freeze-drying step, or requiring precise pipetting of small volumes (manual or via a pipetting machine) or automated high-throughput screening z Several stamps available z Ready to use once reconstituted in the supplied buffer, adding only the matrix, primers and water z Stable for 12 months at room temperature: store and transport at room temperature z Amplification up to 4 kb z The reconstituted Hot Disco Taq master mix (5X) is composed of Hot Disco Taq DNA polymerase, dNTPs, NH4+ buffer and MgCl2 z Available in a "Ready to load" version, containing two dyes (orange G and xylene cyanol FF) and a loading buffer, for monitoring migration Designation Number of 25 µl reactions Part no. € Hot Disco Taq master mix 5X lyophilised 500 257701 NC - 1000 257699 NC - 2500 257700 NC - Hot Disco Taq master mix 5X lyophilised, ready to load 500 257704 NC - 1000 257702 NC - 2500 257703 NC - Specificity Optimisation of MgCl2 concentration and required hybridisation temperature - Standard buffer (Tris-HCl pH 8.5, KCl, 15 mM MgCl2, 1% Tween 20) +++ Yes - Ammonium buffer (Tris-HCl pH 8.5, (NH4)2S04, 15 mM MgCl2, 1% Tween 20) + No - Combination buffer (Tris-HCl, pH 8.7, KCl, (NH4)2SO4, 15 mM MgCl2, 1% Tween 20) ++ Depending on the matrix and primers Designation Number of units (U) Without buffer and without MgCl2 € 10X standard buffer and 25 mM MgCl2 € 10X ammonium buffer and 25 mM MgCl2 € Part no. Part no. Part no. Hot Disco taq 5U/µl, glycerol-free 500 257713 NC - 257725 NC - 257723 NC - 1000 257709 NC - 257716 NC - 257714 NC - 2500 257711 NC - 257719 NC - 257717 NC - 5000 257712 NC - 257722 NC - 257720 NC - Buffers available separately on page 257 Hot Disco Taq Multiplex master mix 2X, ready to use z Master mix 2X ready to use, addition of matrix, primers and water only z Taq inactivated at room temperature, activation at 95 °C for 15 min z Simultaneous amplification of several targets in a single tube z High specificity and sensitivity for high performance z Minimises the risk of artefacts due to possible aspecific polymerisation before the first denaturation step z Possible incorporation of dUTP z The 2X buffer contains Hot Disco Taq DNA polymerase, dNTPS, optimised buffer and 6 mM MgCl2 Designation Number of 50 µl reactions Number of 20 µl reactions Part no. € Hot Disco Taq Multiplex master mix 2X 100 250 257705 NC - 500 1250 257707 NC - 2500 6250 257706 NC - 5000 12500 257708 NC -
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