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Molecular biology Specialty ranges of molecular biology reagents Cytiva Illustra

Cytiva Illustra

Cytiva illustra™ nucleic acid purification

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Cytiva Illustra™ Triple Prep: simultaneous extraction and insulation of DNA, RNA, and proteins from the same sample

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• Kit for a simultaneous extraction and insulation of genomic DNA (gDNA), total RNA, and proteins from the same sample
• Flexible workflow: can isolate any two or all three analytes
• User friendly: minimal change of centrifugation speed, time, and pipetting volume
• Ideal for small samples
• Suited for 10 mg input sample, at different disruption difficulty
• ADN: yield from 6.8 to 22.4 µg, from 10 mg tissue amount
• ARN: yield from 8.1 to 44 µg
• Protein: yield from 510 to 1460 µg
• 90 min procedure, including lysis step

Mini illustra™ tissue and cells genomicPrep Mini Spin kit

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• Illustra tissue and cells genomicPrep Mini Spin kit
• For the rapid extraction of high molecular weight genomic DNA (gDNA) from tissues and cell cultures
• Up to 1.5 µg DNA / mg of tissue from an input tissue sample range of 5 to 50 mg
• Up to 40 µg genomic DNA for 5 millions cells (depending on cell type)
• Total DNA extraction in 90 min
• A 260/ A 280 = 1.8

Mini illustra™ blood genomicPrep Mini Spin kit

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• Illustra blood genomicPrep Mini Spin Kit
• For the rapid extraction and purification of DNA from whole blood, buffy coat, bone marrow, and nucleated red blood cells
• Excellent repeatability and optimal yield (5 to 10 µg DNA from a 200 µl sample)
• Input sample volumes can vary from 50 µl to 1 ml
• DNA purity: A 260/A 280 >1.6
• Total time of preparation: 20 min (includes lysis, purification and desalting)

Mini illustra™ bacteria genomicPrep Mini Spin kit

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• Illustra bacteria genomicPrep Mini Spin Kit
• For the rapid extraction and purification of genomic DNA from gram-positive and gram-negative bacteria
• Total DNA extraction time under 1 h
• Can be used to purify 4 to 8 µg of DNA for Gram + (from 2 x 109bacteria cells)
• Kit designed for input amounts ranging from 1 × 109to 4 × 109bacteria cells
• A260/A280 between 1.7 and 1.9
• Contains Proteinase K

Cytiva illustra GFX™ PCR DNA and Gel Band Purification Kits

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• This kit can be used to purify DNA from reaction volumes up to 100 µl or agarose gel slices up to 900 mg
• Recoveries range from 60% to 80% for DNA fragments from agarose gel to as high as 95% for PCR products from solution
• 99.5% of contaminants removed

Advantages:
• flexible 10 to 50 µl elution volume for different DNA concentration needs;
• Two choices of elution buffer;
• Highly pure DNA is ready for direct use in sequencing, PCR, labeling, restriction enzyme digestion, and cloning.

Purification and concentration of DNA fragments ranging in size from 50 bp to 10 kb, from:
• PCR mixtures;
• DNA-containing agarose gel bands;
• enzyme-based DNA modifications, and restriction enzyme digests

illustra GFX 96 PCR Purification Kit

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• For the purification of up to 96 PCR products (0.1 to 10 kb) simultaneously in as little as 15 min
• Elution volume: 10 - 100 µl
• Validated for use with Macherey-Nagel NucleoVac 96 vacuum manifold
• Typical recoveries are >85% for PCR products 100 bp to 10 kb in length; salt removal typically >= 99%
• Avoids ethanol precipitations and hazardous organic extractions
• Purified DNA is ready for use in most applications, including fluorescent sequencing, microarrays, labeling, hybridization, ligation, and transformation

illustra™ ExoProStar™, ExoProStar™ 1-Step and ExoProStar™ S

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Illustra Shrimp Alkaline Phosphatase and Exonuclease I

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Use alkaline phosphatase and exonuclease to clean PCR reactions before sequencing.

illustra™ GenomiPhi™ DNA Amplification Kit: amplification of the whole genome

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No thermal cycler required!

Sample Weight: 10 ng of genomic DNA

Single Cell Genomiphi™ GELS Illustra

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Enzyme Phi29 DNA polymerase for the amplification of genomic DNA in its entirety, from 1 to 1000 cells.
Suitable for SNP applications, CGH arrays and Next Generation Sequencing.

Illustra™ Templiphi kits

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illustra™ plasmidPrep Cytiva

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Illustra MicroSpin HR columns

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Columns and micro columns

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NAP columns

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• For rapid and efficient purification of DNA and oligonucleotides (10-mers) in less than 15 min
• Purification by gravity flow
• Available in three sizes depending on sample volume: 0.5 ml (NAP-5), 1 ml (NAP-10) and 2.5 ml (NAP-25)

Sephadex G-25 DNA Grade

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• Sephadex powder enables self preparation of columns
• For purification of DNA
• Used in MicroSpin G-25 Columns and NAP Columns

Sephadex G-50 and G-100 DNA Grade

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• Sephadex powder enables self preparation of columns
• For purification of DNA
• Sephadex G-50 DNA Grade is used in illustra AutoSeq G-50 columns, ProbeQuant G-50 Micro Columns, MicroSpin G-50 Columns, and NICK Columns

Cytiva illustra™ PCR kits: Ready-To-Go™ technique

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PuReTaq Ready-To-Go PCR Beads are pre-mixed, predispensed, single-dose reactions optimised for performing standard PCR amplifications.

Cytiva PCR illustra™ kits: Ready-To-Go™ technique

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The RTG (Ready-To-Go) is to provide the mix necessary for the reaction as preformulated, premixed, predispensed beads

• Increased reproducibility
• Minimized pipetting steps
• Reduced the potential for contamination
• Long-term ambient-temperature stable (> 12 months)

Illustra Hot Start Mix RTG beads: for PCR requiring high amplification specificity. The only reagents to be added are water, the DNA to amplify and the primers.

Polymerase Chain Reaction (PCR) is covered by patents held by Roche Molecular Systems and F Hoffmann-La Roche Ltd.

Ready-to-go T4 DNA ligase

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Single-dose lyophilised reaction mix. Ambient-temperature storage. For covalently-linking DNA fragments in 30 min.

Cytiva PCR components

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Taq DNA Polymerase (cloned): 1 unit incorporates 10 nmol of total nucleotides into acid-insoluble material in 30 min at 70ºC, in a total volume of 50 µl.

Cytiva Hot Start MasterMix

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• For demanding PCR applications in which high specificity are essential
• Reduces nonspecific priming
• Kit Contents: Tris-HCl, KCl, MgCl₂, dNTPs, Taq DNA Polymerase, Hot Start Activator protein, additional MgCl₂ tube

Cytiva dATP and dNTP

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• Free from DNase, RNase, and nicking enzyme activity
• Compatible for long range PCR
• Concentration: 100 mm