Molecular Biology - Nucleic Acids Extraction and purification of nucleic acids on columns Extraction and purification of RNA

Extraction and purification of RNA

Cytiva illustra™ nucleic acid purification

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• Isolation d'ARN totaux de haute qualité, pour toute application (exemple : RT-PCR ou qPCR), à partir de divers types d'échantillons
• Préfiltres et DNase inclus
• A partir d'échantillon de culture cellulaire, tissus, bactéries et levures
• Echantillon de tissu jusqu'à 30 mg, pour un rendement de 70 µg
• Echantillon de cellules jusqu'à 5 x 106 cellules, pour un rendement de 70 µg
• Extraction de 6 échantillons en 30 min.
• A 260 / A 280 compris entre 1,8 et 2,2

GeneMATRIX RNA purification kits

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Total RNA and miRNA purification kit.

GENUP blood RNA

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• High quality RNA isolated from 1 ml fresh and frozen, EDTA- or citrate-treated blood
• Fast and simple procedure
• Extraction time: approximately 45 min
• Binding capacity: 20 µg (for 1 ml blood, approx. 8 µg purified total RNA)
• Physical removal of DNA (DNA is bound to a Mini Filter DNA), no DNase treatment
• No β-mercaptoethanol
• High quality RNA suitable for a wide range of different molecular biology applications (RT, northern, RT-qPCR...)

GENUP Plant RNA

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• ARN extraction/purification kit adapted to plant material (up to 100 mg)
• Two lisis buffers to maximize extraction yield
• Fast and simple procedure
• Extraction time: 30 min after homogenization
• Binding capacity: approximately 100 µg RNA
• Physical removal of DNA (DNA is bound to a Mini Filter DNA), no DNase treatment
• No β-mercaptoethanol
• High quality RNA suitable for a wide range of different molecular biology applications (RT, northern, RT-qPCR...)