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Molecular Biology - Nucleic Acids PCR in end point High fidelity DNA polymerases

High fidelity DNA polymerases

Accuris Hot Start DNA Polymerase, High-Fidelity

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• Use in cloning, mutagenesis or DNA chip
• 50 times more accurate than a standard TAQ Polymerase
• Elongation rate: 2kb / min for eukaryotic genomic DNA or cDNAs
• Between 0.5 and 1 U of DNA polymerase required for 25 µl of reaction mixture
• 5X reaction buffer with additives improving the activity and the reaction rate of the enzyme
• Storage 12 months at -20 ° C

PfuPlus! DNA Polymerase

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• Mixture of DNA polymerase Pfu (Pyrococcus furiosus) and a thermostable polymerization activator
• Ultrapure, thermostable and proofreading recombinant polymerase
• Formulation designed to synthesize DNA sequences up to 20 kb
• More than 10 times more accuracy than a standard Taq
• Recommended for high-fidelity PCR, GC-rich sequences or sequences with problematic secondary structures, directed mutagenesis and blunt end cloning
• Can also be used for conventional PCR and high temperature primer extension
• Supplied with a 10X reaction buffer
• Available in 100, 500 and 2500 unit formats
• OnPfuPlus! DNA polymerase: hot start version, stable at room temperature, activation in 10 min at 90 °C

Amplus DNA Polymerase

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• Optimized mixture of thermostable DNA polymerases Taq (Thermus aquaticus), Pfu (Pyrococcus furiosus) and a polymerization activator
• Ultra-pure, thermostable recombinant polymerases. Pfu proofreading.
• Unique composition of the reaction buffer promoting pH stability at high temperature and ensuring high efficiency for long sequence amplifications
• Amplification of genomic DNA sequences from 3 to 25 kb and episomal DNA sequences up to 40 kb
• Particularly suitable for the amplification of eukaryotic genomes
• Ideal for genome mapping and sequencing of long fragments
• Supplied with a 10X reaction buffer
• Available in 100 and 500 unit formats

tiAmplus DNA Polymerase

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• Optimized mixture of thermostable DNA polymerases Taq (Thermus aquaticus), Pfu (Pyrococcus furiosus) and a polymerization activator
• Ultrapure recombinant polymerases, thermostable and hot start. Pfu proofreading
• The hot start property of the polymerases makes them inactive at room temperature allowing the preparation of the reaction mix at room temperature
• Unique composition of the reaction buffer promoting pH stability at high temperature and ensuring high efficiency for long sequence amplifications
• Amplification of genomic DNA sequences from 3 to 25 kb and episomal DNA sequences up to 40 kb
• Particularly suitable for the amplification of eukaryotic genomes
• Ideal for genome mapping and sequencing of long fragments
• Supplied with a 10X reaction buffer
• Available in 100 and 500 unit formats

High fidelity enzyme

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Fidélité 10 fois supérieure à la Taq polymérase.

Accuris high fidelity DNA polymerase

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• Fast expansion: up to 1 kb in 15 seconds
• 100 times more accurate than native Taq polymerase
• 2X formulation for high inhibitor tolerance
• Optimised buffers with additives to improve PCR and increase sensitivity at low copy numbers
• Generates clean ends
• Ideal for cloning, mutagenesis, sequencing