Phosphatases, kinases -

Kinase T4 polynucleotide (PNK)

• Catalyzes the phosphorylation of 5′ hydroxyl termini of double- and single-straned DNA or RNA
• Catalyzes the transfer of the γ-phosphate of ATP to a 5′-OH terminus in DNA or RNA
• The enzyme phosphorylates synthetic linkers and fragments of DNA or RNA prior to ligation
• Used for 5′-end labeling of nucleic acids prior to DNA or RNA sequencing
• Contains 3′-phosphatase activity
• Available in 1000 and 5000 unit formats

Alkaline phosphatase (CIP)

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• Catalyzes the hydrolysis of phosphate monoesters
• Can be used to remove the 5'-P end of DNA or RNA prior to 5' labelling
• Used to remove the 5'-P ends of linearized vectors to prevent their "empty" recircularization during cloning processes
• Compatible with protein dephosphorylation
• Available in 1000 and 5000 unit formats

Thermosensitive bacterial alkaline phosphatase (BAP)

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• Very suitable for diagnostic immunodetection techniques and proteins immunolabelling and nucleic acids transferred on membrane techniques
• Catalyses the release of 5'-P and 3'-P groups of DNA, RNA and nucleotide molecules
• Removes the 5'-P from DNA, RNA, rNTP and dNTP molecules
• Used to remove the 5'-P ends of linearized vectors to prevent their "empty" recircularization during cloning processes
• Resistant to chemical changes and active in a wide variety of reaction buffers
• Can be inactivated by heat for 5 minutes at 70 °C
• Compatible with protein dephosphorylation
• Available in 1000 and 5000 unit formats

Illustra Shrimp Alkaline Phosphatase and Exonuclease I

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Use alkaline phosphatase and exonuclease to clean PCR reactions before sequencing.