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Molecular biology - Proteins Sample preparation Desalting columns

Desalting columns

Cytiva Desalting Columns

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Applications:
• Buffer exchange, desalting
• Clean-up of proteins, peptides, sugars, and more biomolecule
• Removal of small contaminants (salts, dyes, radioactive labels) from samples using gravity flow

Protocol:
• Gel filtration with separation according to molecular weight
• Sephadex™ G-25 media, exclusion limit: > 5000 Mr
• Sephadex™ G-10 media, exclusion limit: > 700 Mr

Vivaspin™ Cytiva concentrators

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• Ultrafiltration columns designed for rapid, non-denaturing concentration and desalting of proteins
• Membrane verticale en polyéthersulfone (PES)
• Polycarbonate (PC) tank